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Product Info Summary
| SKU: | A00139 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Matrix metalloproteinase-9 MMP9 Antibody
SKU/Catalog Number
A00139
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Matrix metalloproteinase-9 MMP9 Antibody (Catalog # A00139). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
MMP9 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Matrix metalloproteinase-9 MMP9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00139)
Host
Rabbit
Contents
MMP9 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
MMP9 antibody was raised against a 16 amino acid peptide near the center of human MMP9. The immunogen is located within amino acids 260 - 310 of MMP9 .
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
MMP9 antibody is human, mouse and rat reactive. At least three isoforms of MMP9 are known to exist; this antibody only recognizes the two longest isoforms.
Reactive Species
A00139 is reactive to MMP9 in Human, Mouse, Rat
Applications
A00139 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
78.458kDa
Background of MMP-9
The matrix metalloproteinase (MMP) family are a family of proteins that are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases (1). MMP9 degrades type IV and V collagens (2) and studies suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow (3) and plays a role in tumor-associated tissue remodeling (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
MMP9 antibody can be used for detection of MMP9 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of MMP9 in mouse lung tissue lysate with MMP9 antibody at 1 μg/ml.
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Immunohistochemistry of MMP9 in human lung tissue with MMP9 antibody at 2.5 μg/mL.
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Immunofluorescence of MMP9 in human lung tissue with MMP9 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For MMP9 (Source: Uniprot.org, NCBI)
Gene Name
MMP9
Full Name
Matrix metalloproteinase-9
Weight
78.458kDa
Superfamily
peptidase M10A family
Alternative Names
92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; EC 3.4.24; EC 3.4.24.35; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase MMP9 CLG4B, GELB, MANDP2, MMP-9 matrix metallopeptidase 9 matrix metalloproteinase-9|macrophage gelatinase|matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)|matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)|type V collagenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MMP9, check out the MMP9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MMP9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Matrix metalloproteinase-9 MMP9 Antibody (A00139)
Hello CJ!
A00139 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Gao X,Qiao X,Xing X,Huang J,Qian J,Wang Y,Zhang Y,Zhang X,Li M,Cui J,Yang Y.Matrix Stiffness-Upregulated MicroRNA-17-5p Attenuates the Intervention Effects of Metformin on HCC Invasion and Metastasis by Targeting the PTEN/PI3K/Akt Pathway.Front Oncol.2020 Aug 19;10:1563.doi:10.3389/fonc.2020.01563.PMID:32974191;PMCID:PMC7466473.
Species: Human
A00139 usage in article: APP:IHC, SAMPLE:HCC CELL, DILUTION:1:50
Gao X,Qiao X,Xing X,Huang J,Qian J,Wang Y,Zhang Y,Zhang X,Li M,Cui J,Yang Y.Matrix Stiffness-Upregulated MicroRNA-17-5p Attenuates the Intervention Effects of Metformin on HCC Invasion and Metastasis by Targeting the PTEN/PI3K/Akt Pathway.Front Oncol.2020
Species: Human
A00139 usage in article: APP:IHC, SAMPLE:HCC CELL, DILUTION:1:50
Li CH,Liu M,Pan LH,Sun Y.ANP reduced Hedgehog signaling-mediated activation of matrix metalloproteinase-9 in gastric cancer cell line MGC-803.Gene.2020 Dec 15;762:145044.doi:10.1016/j.gene.2020.145044.Epub 2020 Aug 7.PMID:32777528.
Species: Human
A00139 usage in article: APP:WB, SAMPLE:MGC-803 CELL, DILUTION:1:400
ACS Appl.Bio Mater.2020,XXXX,XXX,XXX-XXX Publication Date:December 15, 2020 https://doi.org/10.1021/acsabm.0c00927
Species: Human,Mouse
A00139 usage in article: APP:IF, SAMPLE:4T1 CELL AND HUVECS, DILUTION:1:200
Liang Q,Lin Q,Li Y,Luo W,Huang X,Jiang Y,Qin C,Nong J,Chen X,Sooranna SR,Pinhu L.Effect of SIS3 on Extracellular Matrix Remodeling and Repair in a Lipopolysaccharide-Induced ARDS Rat Model.J Immunol Res.2020 Nov 25;2020:6644687.doi:10.1155/2020/6644687.PM
Species: Rat
A00139 usage in article: APP:IHC, SAMPLE:LUNG TISSUE, DILUTION:1:50
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